最近の研究について
研究トピックスの詳細については下記の通りです。
１）翻訳後修飾酵素を用いるタンパク質の部位特異的修飾法
概要：ペプチドやタンパク質中に含まれるグルタミン残基とリジン残基の間を選択的に架橋化する酵素トランスグルタミナーゼを使って、組換えタンパク質のペプチドタグを狙った部位特異的なタンパク質間架橋による二量体化、核酸-タンパク質コンジュゲートの調製等について研究を進めています。
共同研究先（実績含む）：味の素（株）、東京大学、徳島大学
参考論文
1. H. Hirakawa, N. Kamiya, T. Tanaka, T. Nagamune. ‘Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure.’, Protein Eng. Des. Sel., in press. (2007)
2. J. Tominaga, N. Kamiya*, M. Goto. ‘An enzyme-labeled protein polymer bearing pendant haptens.’, Bioconjugate Chem., 18, 860-865 (2007)
3. J. Tominaga, Y. Kemori, Y. Tanaka, T. Maruyama, N. Kamiya*, M. Goto. ‘An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides.’, Chem. Commun., 401-403 (2007)
4. T. Tanaka, N. Kamiya*, T.Nagamune*. ‘N-terminal glycine-specific protein conjugation catalyzed by microbial transglutaminase.’, FEBS Lett., 579, 2092-2096 (2005)
5. T. Takazawa, N. Kamiya, H. Ueda, T. Nagamune. ‘Enzymatic labeling of alkaline phosphatase on antibody variable domain using microbial transglutaminase.’, Biotechnol. Bioeng., 86, 399-404 (2004)
6. T. Tanaka, N. Kamiya, T. Nagamune. ‘Peptidyl linkers for protein heterodimerization catalyzed by microbial transglutaminase’, Bioconjugate Chem., 15, 491-497 (2004)
7. N. Kamiya, T. Tanaka, T. Suzuki, T. Takazawa, S. Takeda, K. Watanabe, T. Nagamune. ‘S-peptide as a potent peptidyl linker for protein crosslinking by microbial transglutaminase from Streptomyces mobaraensis.’, Bioconjugate Chem., 14, 351-357 (2003)
8. N.
Kamiya, T. Takazawa, T, Tanaka, H. Ueda, T. Nagamune. ‘Site-specific
cross-linking of functional proteins by transglutamination.’, Enzyme
Microbial. Technol., 33, 492-496 (2003)
２）翻訳後修飾酵素を用いるタンパク質の部位特異的固定化法
概要：同じくトランスグルタミナーゼを使って、組換えタンパク質のペプチドタグを介した各種固相への配向制御型タンパク質固定化について検討を進めています。
共同研究先（実績含む）：味の素（株）、日本板硝子（株）、富士フイルム（株）
参考論文
1. N. Kamiya*, S. Doi, Y. Tanaka, H. Ichinose, M. Goto. ‘Functional immobilization of recombinant alkaline phosphatases bearing a glutamyl donor substrate peptide of microbial transglutaminase.’, J. Biosci. Bioeng., in press. (2007)
2. Y. Tanaka, Y. Tsuruda, M. Nishi, N. Kamiya*, M. Goto. ‘Exploring enzymatic catalysis at a solid surface: a case study with transglutaminase-mediated protein immobilization.’, Org. Biomol. Chem., 5, 1764-1770 (2007)
3. J. Tominaga, N. Kamiya*, S. Doi, H. Ichinose, T. Maruyama, M. Goto*. ‘Design of a specific peptide tag that affords covalent and site-specific enzyme immobilization catalyzed by microbial transglutaminase.’, Biomacromolecules, 6, 2299-2304 (2005)
4. N. Kamiya*, S. Doi, J. Tominaga, H. Ichinose, M. Goto*. ‘Transglutaminase-mediated protein immobilization to casein nanolayers created on a plastic surface.’, Biomacromolecules, 6, 35-38 (2005)
5. J. Tominaga, N. Kamiya, S. Doi, H. Ichinose, M. Goto. ‘An enzymatic strategy for site-specific immobilization of functional proteins using microbial transglutaminase.’, Enzyme Microbial. Technol., 35, 613-618 (2004)
３）生体触媒の高度利用によるバイオプロダクション
概要：昨今のエネルギー問題の解決には、生体触媒の高度利用ならびにバイオマスの利活用が鍵を握ると言われています。バイオマスの分解・変換におけるキープレーヤーである酵素を実用的な触媒として利用するべく、生化学（タンパク質工学）、有機化学、生物化学工学（酵素工学）をベースに、様々な角度から検討を行っています。
共同研究先（実績含む）：理化学研究所、産業技術総合研究所、（株）豊田中央研究所、東北大学、神戸大学、農学部
３-１）組換え大腸菌を用いる酸化還元酵素触媒の高効率化
参考論文
1. T. Mouri, J. Michizoe, H. Ichinose, N. Kamiya, M. Goto. ‘A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration.’, Appl. Microbiol. Biotechnol., 72, 514-520 (2006)
2. T. Mouri, N. Kamiya, M. Goto. ‘Increasing the catalytic performance of a whole cell biocatalyst harboring a cytochrome P450cam system by stabilization of an electron transfer component.’, Biotechnol. Lett., 28, 1509-1513 (2006)
３-２）非水溶媒（有機溶媒、イオン液体）を反応場とする酵素反応
参考論文
1. K. Shimojo, N. Kamiya, F. Tani, H. Naganawa, Y. Naruta, M. Goto. ‘Extractive solubilization, structural change and functional conversion of cytochrome c in ionic liquids via crown ether complexation.’, Anal. Chem., 78, 7735-7742 (2006)
2. K. Nakashima, T. Maruyama, N. Kamiya, M. Goto. ‘Homogeneous enzymatic reaction in ionic liquids with poly(ethylene glycol)-modified subtilisin.’, Org. Biomol. Chem., 4, 3462-3467 (2006)
3. K. Shimojo, K. Nakashima, N. Kamiya, M. Goto. ‘Crown-ether mediated extraction and functional conversion of cytochrome c in ionic liquids.’, Biomacromolecules, 7, 2-5 (2006)
4. K. Nakashima, T. Maruyama, N. Kamiya, M. Goto. ‘Comb-shaped poly(ethylene glycol)-modified subtilisin Carlsberg is soluble and highly active in ionic liquids.’, Chem. Commun., 4297-4299 (2005)
5. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘Highly enantioselective separation using a supported liquid membrane encapsulating surfactant-enzyme complex.’, J. Am. Chem. Soc., 126, 8622-8623 (2004)
6. H. Ichinose, J. Michizoe, T. Maruyama, N. Kamiya, M. Goto. ‘Electron transfer reaction and functionalization of P450cam system in reverse micelles.’, Langmuir, 20, 5564-5568 (2004)
３-３）超好熱菌由来酵素の応用研究
参考論文
1. H. Hirakawa, N. Kamiya, Y. Kawarabayashi, T.Nagamune. ‘Log P effect of organic solvents on a thermophilic alcohol dehydrogenase.’, Biochim. Biophys. Acta., 1748, 94-99 (2005)
2. H. Hirakawa, N. Kamiya, Y. Kawarabayashi, T. Nagamune. ‘Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1.’, J. Biosci. Bioeng., 97, 202-206 (2004)
４）生物機能を模倣・利用するバイオアセンブリ
概要：自己組織化は、生命活動の様々な場面で普遍的に観察される現象です。当研究室では、特に、界面活性能を有する分子が自己組織的に形成するミセル（水中）あるいは逆ミセルやエマルジョン（非水溶媒中）のバイオ分野への応用について検討しています。前者について、最近、牛乳に大量に含まれるカゼインを化学的に架橋化処理することで、直径約20 nm程度の温度応答性タンパク質性ナノ粒子が簡単に得られることを発見しました。
共同研究先（実績含む）：（財）新世代研究所、東京農工大学
参考論文
1. H. Ichinose, N. Kamiya, M. Goto. ‘Enzymatic redox cofactor regeneration in organic media: Functionalization and application of glycerol dehydrogenase and soluble transhydrogenase in reverse micelles.’, Biotechnol. Prog., 21, 1192-1197 (2005
2. J. Michizoe, H. Ichinose, N. Kamiya, T. Maruyama, M. Goto. ‘Functionalization of the cytochrome P450cam monooxygenase system in the cell-like aqueous compartments of water-in-oil emulsions.’, J. Biosci. Bioeng., 99, 12-17 (2005) [平成18年度 日本生物工学会論文賞 受賞論文]
特許
５）薬理活性ペプチドやタンパク質の経皮デリバリー
概要：最近、薬理活性を有するペプチドや、抗体の様なタンパク質、さらにはsiRNA等のオリゴ核酸が、バイオ医薬として市場に台頭してきました。これらのバイオ医薬は、経口投与されると胃腸で分解されてしまうので、一般的に注射により投与されます。当研究グループでは、バイオ医薬の「塗り薬化」を目指しています。
共同研究先（実績含む）：ASPION（株）、東レ（株）先端技術研究所、医学部
参考論文
1. H. Piao, N. Kamiya, A. Hirata, T. Fujii, I. Shimizu, S. Ito, M. Goto. ‘A novel solid-in-oil nanosuspension for transdermal delivery of diclofenac sodium.’, Pharm. Res., in press.
2. H. Yoshiura, M. Hashida, N. Kamiya, M. Goto. ‘Factors affecting protein release behavior from surfactant-protein complexes under physiological conditions.’, Int. J. Pharm., 338, 174-179 (2007)
3. H. Piao, N. Kamiya, J. Watanabe, H. Yokoyama, A. Hirata, T. Fujii, I. Shimizu, S. Ito, M. Goto. ‘Oral delivery of diclofenac sodium using a novel solid-in-oil suspension.’, Int. J. Pharm., 313, 159-162 (2006)
4. E. Toorisaka, M. Hashida, Y. Kokazu, N. Kamiya, H. Ono, K. Arimori, M. Goto. ‘An enteric-coated dry emulsion formulations for oral insulin delivery.’, J. Control. Rel., 107, 91-96 (2005)
5. N. Kamiya, A. M. Klibanov, ‘Controling the rate of protein release using polyelectrolyte complexes.’, Biotechnol. Bioeng., 82, 590-594 (2003)
6. E. Toorisaka, H. Ono, K. Arimori, N. Kamiya, M. Goto. ‘Hypoglycemic effect of surfactant-coated insulin solubilized in a novel solid-in-oil-in-water (S/O/W) emulsion.’, Int. J. Pharm., 252, 271-274 (2003)
研究概要図
主な研究内容の俯瞰図を、化学工学会九州支部HP内に掲載しています。
http://www.chem-eng.kyushu-u.ac.jp/chen_kyushu/q-net/seeds/kamiya.html
研究室内外共同研究
そのほか、当研究室ではバイオと界面工学に関連する様々な研究が行われています。基本的には、それぞれのスタッフが独立して研究を行いますが、研究領域がオーバーラップする部分が多い研究については、共同研究形式で研究が行われています。また、産学官共同研究も積極的に推進しています。
参考論文
1. T. Maruyama, Y. Shimada, H. Matsushita, N. Kamiya, M. Goto. ‘Proteins and protein-rich biomass as environmental-friendly adsorbents selective for precious metal ions.’, Env. Sci. Technol., 41, 1359-1364 (2007)
2. T. Maruyama, T. Shinohara, T. Hosogi, H. Ichinose, N. Kamiya, M. Goto. ‘Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching.’, Anal. Biochem., 354, 8-14 (2006)
3. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘A supported liquid membrane encapsulating a surfactant-lipase complex for selective separation of organic acids.’, Chem. Eur. J., 11, 1163-1170 (2005)
4. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘Optical resolution of various amino acids using a supported liquid membrane encapsulating a surfactant-protease complex.’, Langmuir, 20, 5564-5568 (2005)
5. K. Araki, T. Maruyama, N. Kamiya, M. Goto. ‘Metal ion-selective membrane prepared by surface molecular imprinting.’, J. Chromatography B, 818, 141-145 (2005)
6. J. Michizoe, H. Ichinose, N. Kamiya, T. Maruyama, M. Goto. ‘Biodegradation of phenolic environmental pollutants by a surfactant-laccase complex in organic media.’, J. Biosci. Bioeng., 99, 642-647 (2005)
7. H. Ichinose, M. Kitaoka, N. Okamura, T. Maruyama, N. Kamiya, M.Goto. ‘Detection of single-base mutations by fluorogenic ribonuclease proteiction assay.’, Anal. Chem., 21, 1192-1197 (2005)
8. T. Maruyama, T. Takata, H. Ichinose, N. Kamiya, H. Kuma, N. Hamasaki, H. Morita, M. Goto. ‘Detection of point mutations in the HBV polymerase gene using a fluorescence intercalator in reverse micelles.’, Biotechnol. Prog., 21, 575-579 (2005)
9. T. Maruyama, T. Shinohara, H. Ichinose, M. Kitaoka, N. Okamura, N. Kamiya, M.Goto. ‘Mutation detection in DNA oligonucleotides based on a guanine quenching method coupled with enzymatic digestion of single-stranded DNA.’, Biotechnol. Lett., 27, 1349-1354 (2005)
10. M. Sakono, T. Maruyama, N. Kamiya, M. Goto. ‘Direct refolding of inclusion bodies using reversed micelles.’, Biotechnol. Prog., 20, 1783-1787 (2004)
11. L.-C. Park, T. Maruyama, N. Kamiya, M. Goto, H. Kuma, N. Hamasaki. ‘Mutation detection in the drug-resistant hepatitis B virus polymerase gene using nanostructured reverse micelles.’, Anal. Sci., 20, 1609-1611 (2004)
12. T. Maruyama, T. Kaji, T. Ohkawa, K. Sotowa, H. Matsushita, F. Kubota, N. Kamiya, K. Kusakabe, M. Goto. ‘Intermittent partition walls promote solvent extraction of metal ions in a microfluidic device.’, Analyst, 129, 1008-1013 (2004)
13. T. Maruyama, H. Matsushita, J. Uchida, F. Kubota, N. Kamiya, M. Goto. ‘Liquid membrane operations in a microfluidic device for selective separation of metal ions.’, Anal. Chem., 76, 4495-4500 (2004)
14. T. Maruyama, H. Yamamura, T. Kotani, N. Kamiya, M. Goto. ‘Poly(ethylene glycol)-lipase complex highly active and enantioselective in ionic liquids.’, Org. Biomol. Chem., 2, 1239-1244 (2004)
15. J. Tominaga, J. Michizoe, N. Kamiya, H. Ichinose, T. Maruyama, M. Goto. ‘Factors affecting oxidative activity of laccase against biphenyl derivatives in an aqueous-organic homogeneous system.’, J. Biosci. Bioeng., 98, 14-19 (2004)
16. S. Takeda, N. Kamiya, T. Nagamune. ‘Rational design of a protein-based molecular device consisting of blue fluorescent protein and zinc protoporphyrin IX incorporated into a cytochrome b562 scaffold.’, Biotechnol. Lett., 26, 121-125 (2004)
17. T. Maruyama, T. Kotani, H. Yamamura, N. Kamiya, M. Goto. ‘Poly(ethylene glycol)-lipase complexes catalytically active in fluorous solvents.’, Org. Biomol. Chem., 2, 524-527. (2004)
18. J. Michizoe, Y. Uchimura, H. Ichinose, T. Maruyama, N. Kamiya, H. Wariishi, S. Furusaki, M. Goto. ‘Activation of manganese peroxidase in an organic medium using a mediator.’, Biochem. Eng. J., 19, 43-46 (2004)
19. M. Sakono, T. Maruyama, N. Kamiya, M. Goto. ‘Refolding of denatured carbonic anhydrase B by nanostructured reversed micelles.’, Biochem. Eng. J., 19, 217-220 (2004)
20. B. Lee, N. Kamiya, S. Machida, Y. Yamagata, K. Horie, T. Nagamune. ‘Fabrication of a protein film by electrosplay deposition method and investigation of photochemical properties by persistent spectral hole burning.’, Biomaterials, 24, 2045-2051 (2003)
21. S, Takeda, N. Kamiya, T. Nagamune. ‘A novel protein-based heme sensor consisting of green fluorescent protein and apocytochrome b562.’, Anal. Biochem., 317, 116-119 (2003)
22. T. Zako, K. Ayabe, N. Aburatani, N. Kamiya, A. Kitayama, H. Ueda, T. Nagamune. ‘Luminescent and substrate binding activities of firefly luciferase N-terminal domain.’, Biochim. Biophys. Acta., 1649, 183-189 (2003)
23. S. Yamaguchi, T. Mannen, T. Zako, N. Kamiya, T. Nagamune. ‘Measuring adsorption of a hydrophobic probe with a surface plasmon resonance sensor to monitor conformational changes in immobilized proteins.’, Biotechnol. Prog., 19, 1348-1354 (2003)
24. T. Maruyama, T. Takata, H. Ichinose, L.-C. Park, N. Kamiya, M. Goto. ‘Simple detection of point mutations in DNA oligonucleotides using SYBR Green I.’, Biotechnol. Lett., 25, 1637-1641 (2003)
25. T. Maruyama, J. Uchida, T. Ohkawa, T. Futami, K. Katayama, K. Nishizawa, K. Sotowa, F. Kubota, N. Kamiya, M. Goto. ‘Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system.’, Lab. Chip, 3, 315-319 (2003)
26. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘Enzyme-facilitated enantioselective transport of (S)-ibuprofen through a supported liquid membrane based on ionic liquids.’, Chem. Commun., 2926-2927 (2003)
27. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘Use of ionic liquids in a lipase-facilitated supported liquid membrane.’, Biotechnol. Lett., 25, 805-808 (2003)
28. E. Miyako, T. Maruyama, N. Kamiya, M. Goto. ‘Transport of organic acids through a supported liquid membrane driven by lipase-catalyzed reactions.’, J. Biosci. Bioeng., 96, 370-374 (2003)
29. J. Michizoe, Y. Uchimura, T. Maruyama, N. Kamiya, M. Goto. ‘Control of water content by reverse micellar solutions for peroxidase catalysis in a water-immiscible organic solvent.’, J. Biosci. Bioeng., 95, 425-427 (2003)
30. T. Maruyama, S. Noda, N. Kamiya, M. Goto. ‘Ring-opening polymerization of lactones catalyzed by surfactant-coated lipases in organic solvents.’, J. Chem. Eng. Japan, 36, 307-312 (2003)
31. H. Hirakawa, N. Kamiya, T. Yata, T. Nagamune. ‘Regioselective reduction of a steroid in a reversed micellar system with enzymatic NADH-regeneration.’, Biochem. Eng. J., 16, 35-40 (2003)
２００２年以前の論文リスト
1.
N. Kamiya, T. Nagamune.
‘Effect of water activity control on the catalytic performance of surfactant-Arthromyces ramosus peroxidase complex
in toluene.’ Biochem. Eng. J., 10,
55-59 (2002)
2.
M. Shimizu, N. Kamiya, A. Kitayama, T. Nagamune.
‘Self-assembly of electron transport protein using oligonucleotide
hybridization.’, Colloid. Surface. B, 25, 69-79 (2002)
3.
N. Kamiya, Y. Okimoto,
Z. Ding, H. Ohtomo, M. Shimizu, A. Kitayama, H. Morii, T. Nagamune. ‘How does
heme axial ligand deletion affect the structure and the function of cytochrome b562?’ Protein Eng., 14, 415-419 (2001)
4.
N. Kamiya, T. Ogawa,
T. Nagamune. ‘Enhancement of apparent thermostability of lipase from Rhizopus sp. by the treatment with
microbial transglutaminase.’ Biotechnol.
Lett., 23, 629-1632 (2001)
5.
S. Takeda, N. Kamiya, R. Arai, T. Nagamune.
‘Design of an artificial light-harvesting unit by protein engineering:
cytochrome b562-green
fluorescent protein chimera.’, Biochem.
Biophys. Res. Commun., 289, 299-304 (2001)
6.
R. Arai, H. Ueda, A. Kitayama, N. Kamiya,
T. Nagamune. ‘Design of the linkers which effectively separate domains of a
bifunctional fusion protein.’, Protein
Eng., 14, 529-532 (2001)
7.
N. Kamiya, M. Inoue,
M. Goto, N. Nakamura, Y. Naruta. ‘Catalytic and structural properties of
surfactant-horseradish peroxidase complex in organic media.’ Biotechnol. Prog., 16, 52-58 (2000)
8.
N. Kamiya, H.
Kasagi, K. Kusunoki, M. Goto. ‘Enantioselective recognition of secondary
alcohols by surfactant-coated lipases in nonaqueous media.’, Biotechnol. Bioeng., 65, 227-232 (1999)
9.
N. Kamiya, M.
Goto, S. Furusaki. ‘Surfactant-histidine-heme ternary complex as a simple
artificial heme enzyme in organic media.’, Biotechnol.
Bioeng., 64, 502-506 (1999)
10. N.
Kamiya, M. Goto. ‘Preparation of surfactant-coated
lipases utilizing molecular imprinting technique.’, J. Ferment. Bioeng., 85, 237-239 (1998)
11. L.
Iskandar, T. Ono, N. Kamiya, M. Goto, F. Nakashio, S. Furusaki.
‘Catalytic properties of novel reversed micellar system on transesterification
in organic media.’, Biochem. Eng. J.,
2, 29-33 (1998)
12. N.
Kamiya, M. Goto. ‘How is enzymatic selectivity of
menthol esterification catalyzed by surfactant-coated lipase determined in
organic media?’, Biotechnol. Prog.,
13, 488-492 (1997)
13. N.
Kamiya, S. Furusaki, M. Goto. ‘Peroxidase activity and
stability of surfactant-heme complex in nonaqueous media.’, Biotechnol. Lett., 19, 1015-1018 (1997)
14. N.
Kamiya, S. Okazaki, M. Goto. ‘Surfactant-horseradish
peroxidase complex catalytically active in anhydrous benzene.’, Biotechnol. Tech., 11, 375-378 (1997)
15. S.
Noda, N. Kamiya, M. Goto, F. Nakashio. ‘Enzymatic polymerization catalyzed
by surfactant-coated lipases in organic media.’, Biotechnol. Lett., 19, 307-309 (1997)
16. S.
Okazaki, N. Kamiya, M. Goto, F. Nakashio. ‘Enantioselective esterification
of glycidol by surfactant-lipase complexes in organic media.’, Biotechnol. Lett., 19, 541-543 (1997)
17. S.
Okazaki, N. Kamiya, K. Abe, M. Goto, F. Nakashio. ‘Novel preparation method
for surfactant-lipase complexes utilizing water in oil emulsions.’, Biotechnol. Bioeng., 55, 455-460 (1997)
18. S.
Okazaki, N. Kamiya, M. Goto. ‘Application of novel preparation method
for surfactant-protease complexes catalytically active in organic media.’, Biotechnol. Prog., 13, 551-556 (1997)
19. N.
Kamiya, E. Murakami, M. Goto, F. Nakashio. ‘Effect of using
co-solvent in the preparation of surfactant-coated lipases on catalytic activity
in organic media.’, J. Ferment. Bioeng.,
82, 37-41 (1996)
20. M.
Goto, S. Noda, N. Kamiya, F. Nakashio. ‘Enzymatic resolution of racemic ibuprofen
by surfactant-coated lipases in organic media.’, Biotechnol. Lett., 18, 839-844 (1996)
21. N.
Kamiya, M. Goto, F. Nakashio. ‘Surfactant-coated
lipase suitable for the enzymatic resolution of menthol as a biocatalyst in
organic media.’, Biotechnol. Prog.,
11, 270-275 (1995)
22. M.
Goto, M. Miyata, N. Kamiya, F. Nakashio. ‘Novel Surfactant-coated
enzymes immobilized in poly(ethylene glycol) microcapsules.’, Biotechnol. Tech., 9, 81-84 (1995)
23. M.
Goto, M. Goto, N. Kamiya, F. Nakashio. ‘Enzymatic interesterification of
triglyceride with surfactant-coated lipase in organic media.’, Biotechnol. Bioeng., 45, 27-32 (1995)
24. M.
Goto, N. Kamiya, M. Miyata, F. Nakashio. ‘Enzymatic esterification by
surfactant-coated lipase in organic media.’, Biotechnol. Prog., 10, 263-268 (1994)